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KMID : 0355220080330010035
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Journal of Korean Academy Oral Medicine
2008 Volume.33 No. 1 p.35 ~ p.40
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Effect of Glucose at High Concentrations on the Integrin and Cathepsin Expression of the Cultured Human Periodontal Ligament Cells
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Kim Bang-Soo
Shin Je-Won
Hong Jung-Pyo
Jue Seong-Suk
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Abstract
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This experiment was designed to clarify the effect of high concentrations of extracellular glucose on the periodontal ligament cells. The cells were incubated for 24 and 48 hours with α-MEM including 1,000 ㎎/L (control group) and 4,500 ㎎/L (experimental group) of glucose. Then, the expressions of α5 integrin, cathepsin-B and -L were examined using RT-PCR method. The results were as follows:
1. α5 integrin expression was increased after incubation in high glucose media.
2. Cathepsin-B expression was increased after the 24-H incubation with high glucose media, but was decreased after the 48-H incubation.
3. The expression of cathepsin-L was decreased by the high glucose media.
Theses results suggest that extracellular glucose at high concentration may be attributed to delayed wound healing in diabetes patients through increase in α5 integrin and decrease in cathepsins, which lead to accumulation of extracellular matrices and adhesion molecules.
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KEYWORD
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Integrin, Cathepsin, Glucose, Periodontal ligament cells
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